pig kidney epithelial cells  (ATCC)

Journal: Journal of Lipid Research

Article Title: Dyslipidemia-induced renal fibrosis related to ferroptosis and endoplasmic reticulum stress

doi: 10.1016/j.jlr.2024.100610

Figure Lengend Snippet: Ferrostatin-1 rescued palmitic acid and oxidized-LDL–induced mitochondrial damage in PK1 cells. A and C: Mito-SOX staining showed elevated reactive oxygen species production in PA, ox-LDL, and PA + ox-LDL cells, which Fer-1 reversed. B and D: Tetramethylrhodamine ethyl ester (TMRE) staining showed decreased mitochondrial membrane potential in the PA, ox-LDL, and PA + ox-LDL groups, which Fer-1 recovered. E: PA, ox-LDL, and PA + ox-LDL treatment significantly decreased GSH level in PK1 cells, which Fer-1 improved. F: PA, ox-LDL, and PA + ox-LDL treatment decreased ATP production in PK1 cells, and Fer-1 only ameliorated PA-mediated ATP decrease. ns: no significance, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 between groups; # P < 0.05, ## P < 0.01, and ### P < 0.001 over graph bars compared with DMSO group; ∭ P < 0.001 over graph bars compared with Fer-1 group. PK1, pig kidney epithelial cell.

Article Snippet: Pig kidney epithelial cells (LLC-PK1, ATCC, Manassas, VA) were incubated with vehicle (2% EtOH and 20% BSA in PBS, control), PA ( ) (0.5 mM in vehicle, Sigma), oxidized-LDL ( ) (ox-LDL, 15 μg/ml in vehicle, Sigma), or their combination (PA + ox-LDL) for 24 h in an attempt to mimic the in vivo microenvironments of ND, HFD, PCSK9-GOF/ND, and PCSK9-GOF/HFD, respectively.

Techniques: Staining, Membrane

Journal: Journal of Lipid Research

Article Title: Dyslipidemia-induced renal fibrosis related to ferroptosis and endoplasmic reticulum stress

doi: 10.1016/j.jlr.2024.100610

Figure Lengend Snippet: Ferrostatin-1 rescued palmitic acid–induced ferroptosis in PK1 cells. A, B: Oil-red-O staining showed increased intracellular lipid droplets in the PA, ox-LDL, and PA + ox-LDL groups, which Fer-1 significantly blunted. C, D: FerroOrange staining detected increased intracellular ferrous ions level in the PA-treated and PA + ox-LDL–treated PK1 cells. E, F: Flow cytometry using C11-BODIPY 581/591 probe showed that PA, ox-LDL, and PA + ox-LDL treatment increased oxidized lipids in PK1 cells, which Fer-1 decreased. Scale bar = 20 μm ns: no significance, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 between groups; # P < 0.05, ## P < 0.01, and ### P < 0.001 over graph bars compared with DMSO group; ∬ P < 0.01, ∭ P < 0.001 over graph bars compared with Fer-1 group. Ox-LDL, oxidized-LDL; PK1, pig kidney epithelial cell.

Article Snippet: Pig kidney epithelial cells (LLC-PK1, ATCC, Manassas, VA) were incubated with vehicle (2% EtOH and 20% BSA in PBS, control), PA ( ) (0.5 mM in vehicle, Sigma), oxidized-LDL ( ) (ox-LDL, 15 μg/ml in vehicle, Sigma), or their combination (PA + ox-LDL) for 24 h in an attempt to mimic the in vivo microenvironments of ND, HFD, PCSK9-GOF/ND, and PCSK9-GOF/HFD, respectively.

Techniques: Staining, Flow Cytometry